Federal regulators are investigating Tesla’s autopilot feature after a fatal crash involving a tractor trailer and one of its Model S cars.

The U.S. National Highway Traffic Safety Administration opened the investigation after a man was killed while driving a Model S with the self-driving mode engaged.

“This is the first known fatality in just over 130 million miles where Autopilot was activated,” Tesla said in a statement Thursday. It called the incident a “tragic loss.”

The car was on a divided highway when a tractor trailer made a left turn in front of the Tesla at an intersection. “Neither Autopilot nor the driver noticed the white side of the tractor trailer against a brightly lit sky, so the brake was not applied,” Tesla said.

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